aureus using high-resolution melting (HRM) analysis of the spa polymorphic region X. We describe here a method for genotyping S. The real-time PCR platform supports genotyping methods that are single step and closed tube and potentially can be carried out simultaneously with diagnosis. The staphylococcal protein A (spa) locus of Staphylococcus aureus contains a complex repeat structure and is commonly used for single-locus sequence-based genotyping.
